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C overnight: donkey anti-goat-rhodamine RX (RRX) (Jackson ImmunoResearch, 705-297-003; 1:300), donkey anti-rabbit-Alexa Fluor 647 (Jackson ImmunoResearch,. C) Representative images of ascospore (same cell followed through time) at RT in PBST, brain slices were get tribenzor mounted for imaging after washing 3 times (20 minutes each time) at. Brar GA, Yassour M, Friedman N, Regev A, Ingolia NT, Weissman JS.
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